An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.
Electroporation of zygotes represents a rapid alternative to the elaborate pronuclear injection procedure for CRISPR/Cas9-mediated genome editing in mice.However, current protocols for Bath Caddy electroporation either require the investment in specialized electroporators or corrosive pre-treatment of zygotes which compromises embryo viability.Here